Description

With a Kcat/Km of 3.3 x 10⁶ M^-1s^-1 this fluorogenic caspase-1 substrate is cleaved 50-fold more efficiently than CASP-010. This phenomena shows that the optimal tetrapeptide recognition sequence for caspase-1 is Trp-Glu-His-Asp and not Tyr-Val-Ala-Asp.

SEQUENCE: Ac-Trp-Glu-His-Asp-AMC (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Ac-WEHD-AMC

MOLECULAR FORMULA: C₃₈H₄₀N₈O₁₁

MOLECULAR WEIGHT: 784.8

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [189275-74-9]

SYNONYMS:

RESEARCH AREA: Apoptosis

REFERENCES:
T.A. Rano et al., Chem. Biol. 4, 149 (1997)

SKU(s): CASP-003A

KEYWORDS: dye-labeled