Description

Highly fluorescent substrate for the determination of caspase-1 (ICE) and caspase-1-like enzyme activities. Cleavage of this substrate at position P1 Asp causes continuous fluorescent assay monitored at an emission wavelength of 393 nM. Furthermore, caspase-3 (apopain, CPP-32) is only weakly active on this substrate. Both caspase-1 and caspase-3 are involved in apoptosis.

SEQUENCE: MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH

ONE-LETTER SEQUENCE: Mca-YVADAP-Lys(DNP)

MOLECULAR FORMULA: C₅₃H₆₄N₁₀O₁₉

MOLECULAR WEIGHT: 1145.15

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [189696-01-3]

SYNONYMS: Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH

RESEARCH AREA: Apoptosis

REFERENCES:
M. Enari et al., Nature, 380, 723 (1996)

SKU(s): CASP-027A, CASP-027B, CASP-027C

KEYWORDS: dye-labeled, FRET Substrate