Description

AMC (7-amino-4-methylcoumarin) derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm. Caspase-3 and caspase-7 substrates

SEQUENCE: Z-Asp-Glu-Val-Asp-AMC

ONE-LETTER SEQUENCE: Z-DEVD-AMC

MOLECULAR FORMULA: C₃₆H₄₁N₅O₁₄

MOLECULAR WEIGHT: 767.8

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Apoptosis

REFERENCES:
Koeplinger KA, et al. (2000) Protein Expr Purif 18, 378-87
Garcia-Calvo M, et al. (1998) J Biol Chem 273, 32608-13
Thornberry NA, et al. (1997) J Biol Chem 272, 17907-11
Rano TA, et al. (1997) Chem Biol 4, 149-55
Margolin N, et al. (1997) J Biol Chem 272, 7223-8
Mizushima N, et al. (1996) FEBS Lett 395, 267-71
Faubion WA, et al. (1999) J Clin Invest 103, 137-45.

SKU(s): CASP-085A, CASP-085B

KEYWORDS: dye-labeled