Description

pNA (4-nitroaniline)-derived caspase substrates widely used for various caspase activities and colorimetric detection. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm. Caspase-9 substrate.

SEQUENCE: Ac-Leu-Glu-His-Asp-pNA (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Ac-LEHD-pNA

MOLECULAR FORMULA: C₂₉H₃₈N₈O₁₁

MOLECULAR WEIGHT: 674.7

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Apoptosis

REFERENCES:
Grutter MG (2000) Curr Opin Struct Biol 10, 649-55
Gastman BR (2001) Head Neck 23, 409-25
Grutter MG (2000) Curr Opin Struct Biol 10, 649-55
Stennicke HR and Salvesen GS (1999)Cell Death Differ 6, 1054-9
Stennicke HR and Salvesen GS (1998) Biochim Biophys Acta 1387, 17-31
Thornberry NA and Lazebnik Y (1998) Science 281, 1312-6
Talanian RV, et al. (1997) . J Biol Chem 272, 9677-82.
Fassy F, et al. (1998) Eur J Biochem 253, 76-83, Datta R, et al. (1996) Blood 88, 1936-43
Koeplinger KA, et al. (2000) Protein Expr Purif 18, 378-87

SKU(s): CASP-091A, CASP-091B

KEYWORDS: dye-labeled