Description

A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.

SEQUENCE: Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Ac-DED(EDANS)-EE-Abu-psi-(COO)AS-K(DABCYL)-NH2

MOLECULAR FORMULA: C₆₈H₈₉N₁₅O₂₅S₁

MOLECULAR WEIGHT: 1548.6

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS: Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2, Ac-DE-D(Edans)-EE-Abu-epsilon-[COO]-AS-K(Dabcyl)-NH2

RESEARCH AREA: Antimicrobial

REFERENCES:
Lin, C. et al. J. Biol. Chem. 10, 1074 (2004)
Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999)
Liu, Y. et al. Anal. Biochem. 267, 331 (1999)
Taliani, M. et al. Anal. Biochem. 240, 60 (1996)

SKU(s): HCVP-001A, HCVP-001B

KEYWORDS: dye-labeled, FRET Substrate