Description

Sensitive substrate for continuous assays and in situ determination of the matrix metalloproteinase activity. When cleaved at the Gly-Leu bond it separates the highly fluorescent Mca group from the efficient 2,4-dinitrophenyl quencher and results in the increase of fluorescence intensity. The kcat/km value for the punctuated metalloproteinase (MMP-7) and for gelatinase (MMP-2), e.g., are 1.7 x 105 and 6.3 x 10⁵ M^-1s^-1, respectively.

SEQUENCE: MCA-Pro-Leu-Ala-Nva-Dap(DNP)-Ala-Arg-NH2 (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Mca-PLA-Nva-Dap(Dnp)-AR-NH2

MOLECULAR FORMULA: C₄₉H₆₈N₁₄O₁₅

MOLECULAR WEIGHT: 1093.16

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [256394-92-0]

SYNONYMS: Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH₂

RESEARCH AREA: Cancer

REFERENCES:
G. Murphy, et al., J. Biol. Chem. 269, 6632 (1994)

SKU(s): MMPS-006A, MMPS-006B

KEYWORDS: dye-labeled, FRET Substrate