Description

This bisamide peptide rhodamine 110 (R110) derivative is a sensitive and selective substrate for assaying proteases in solution or inside live cells. This substrate contains an Z-protected arginine residue covalently linked to each of R110’s amino groups. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is first converted to the fluorescent monoamide and then to R110, with a further increase in fluorescence.

SEQUENCE: (Z-Arg)₂-R110 (hydrochloride salt)

ONE-LETTER SEQUENCE: (Z-R)2-R110

MOLECULAR FORMULA: C₄₈H₅₀N₁₀O₉

MOLECULAR WEIGHT: 910.99

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Inflammation

REFERENCES:
Biol Chem Hoppe-Seyler 373, 433 (1992)
Nature 361, 274 (1993)

SKU(s): SUBS-003A, SUBS-003B

KEYWORDS: dye-labeled