Description

TACE FRET substrate I that is derived from the cleavage sequence of pro-TNFa recognized by the TNFa-cleaving enzyme (TACE). TNFa is presumably shed from cell membranes by TNFa-cleaving enzyme (TACE). Incubation of the TACE substrate with recombinant human TACE gives a specific cleavage to restore the quenched fluorescence. The substrate is widely used to screen inhibitors of TNF-convertase.

SEQUENCE: DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Dabcyl-LAQAVRSSSR-EDANS

MOLECULAR FORMULA: C₇₀H₁₀₄N₂₂O₁₈S₁

MOLECULAR WEIGHT: 1573.80

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [396716-14-6]

SYNONYMS: DABCYL-TNF-α-EDANS (-4 to +6) (human)

RESEARCH AREA: Apoptosis

REFERENCES:
A.J. H.Gearing et al., Nature, 370, 555 (1994)
K.M. Mohler et al., Nature, 370, 218 (1994)
Becker BF, et al. Biol Chem, 383, 1821-6 (2002)
Mohler KM, et al., Nature, 370, 218-20. (1994)

SKU(s): SUBS-014A, SUBS-014B

KEYWORDS: dye-labeled, FRET Substrate