• IL-1b is an innate immune sensor of microbial proteolysis.

    LaRock, Christopher N., et al. Science Immunology 100.200: 300 (2016).

    "Internally quenched peptides of the sequences of IFFDTWDNE, TWDNEAYVH, EAYVHDAPV, and HDAPVRSLN, corresponding to amino acids 103 to 111, 107 to 115, 111 to 119, and 115 to 123 of the reference human pro–IL-1b sequence (UniProt: P01584), were labeled on the N terminus with Mca and on the C terminus with Lys-Dnp (CPC Scientific)."

    Published On: August 19th, 2016Categories: Citations, FRET Substrates
  • Cysteine and Aspartyl Proteases Contribute to Protein Digestion in the Gut of Freshwater Planaria.

    Goupil, Louise S., et al. PLoS Negl Trop Dis 10.8 (2016): e0004893.

    "Protease activity in regurgitant from three replicate tanks were compared using a mixture of 7 internally quenched fluorescent substrates available from CPC Scientific, Sunnyvale, California (Table 1) [AMYD-112, AMYD-114, AMYD-109, MMPS-024, SUBS-017, AMYD-111, CASP-060]. These substrates were chosen based on their diverse sequence composition to enable detection of multiple protease classes."

    Published On: August 8th, 2016Categories: Citations, FRET Substrates