• A covalent BTK ternary complex compatible with targeted protein degradation.

    Schiemer, J., Maxwell, A., Horst, R., Liu, S., Uccello, D.P., Borzilleri, K., Rajamohan, N., Brown, M.F. and Calabrese, M.F. Nature Communications 14, no. 1 (2023): 1189.

    • Discovery Sciences, Pfizer Worldwide Research and Development, Groton, CT, USA

    After 3 h the resin was washed with binding buffer until no protein was detected, followed by elution with 0.2 mg ml−1 FLAG peptide DYKDDDDK (CPC Scientific Peptide company)

    Published On: March 1st, 2023Categories: Citations
  • Discovery of potent and orally bioavailable macrocyclic peptide-peptoid hybrid CXCR7 modulators.

    Boehm, M., Beaumont, K., Jones, R.M., Kalgutkar, A.S., Zhang, L., Atkinson, K., Bai, G., Brown, J.A., Eng, H., Goetz, G.H. and Holder, B.R. Journal of Medicinal Chemistry (2017).

    1. Pfizer Worldwide Research & Development, Cambridge, Massachusetts 02139, United States
    2. Pfizer Worldwide Research & Development, Groton, Connecticut 06340, United States

    The chemokine receptor CXCR7 is an attractive target for a variety of diseases. While several small-molecule modulators of CXCR7 have been reported, peptidic macrocycles may provide advantages in terms of potency, selectivity, and reduced off-target activity

    Published On: October 18th, 2017Categories: Citations, Peptoids
  • Multivalent peptidic linker enables identification of preferred sites of conjugation for a potent thialanstatin antibody drug conjugate.

    Puthenveetil, Sujiet, et al. PloS One 12.5 (2017): e0178452.

    1. Worldwide Medicinal Chemistry, Pfizer Global R&D, Groton, Connecticut, United States of America.
    2. Pfizer Oncology Research, Pearl River, NY, United States of America.

    "Peptide-payload conjugate (7) was prepared by reacting 2mM aizoacetyl-Ser-Lys-Gly-Ser-Lys (CPC scientific, inc. Sunnywale, CA) with 8 mM NHS-ester payload [19] in 50% Dimethyl sulfoxide (DMSO), 50 mM borate buffer pH 8.5 for 2 h at 37°C."

    Published On: May 30th, 2017Categories: Citations, Click Peptides
  • Improved variants of SrtA for site-specific conjugation on antibodies and proteins with high efficiency.

    Chen, Long, et al. Scientific Reports 6 (2016).

    1. Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Peking University, Beijing 100871, China
    2. Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA
    3. School of Life Sciences, Nanjing University, China
    4. Peking-Tsinghua Center for Life Sciences, Beijing, China

    "The Biotin-C6-LELPETGG-NH2, GGGY-Lys(Biotin)-NH2, and GGG-Lys(N3)-NH2 reagents were synthesized by CPC Scientific."

    Published On: August 18th, 2016Categories: Citations, Click Peptides, Unnatural Amino Acids
  • Identification of a novel BODIPY minihepcidin tool for the high content analysis of Ferroportin (SLC40A1) pharmacology.

    Skerratt, Sarah E., Sian Humphreys, Rita Ferreira, Csilla Jorgensen, Joe Warmus, Lei Zhao, Xiaohe Tong, and Sarah A. Nickolls. MedChemComm 7, no. 8 (2016): 1564-1571.

    1. Pfizer Neusentis, The Portway Building, Granta Park, Cambridge, UK.
    2. Pfizer Worldwide Research & Development, Eastern Point Road, Groton, Connecticut 06340, USA.
    3. CPC Scientific Inc., 1245 Reamwood Avenue, Sunnyvale, CA 94089, USA.

    "antipeptide (EGVYVHPV), angiotensin II, human (DRVYIHPF), and isotopically (13C) labeled heptapeptide (AAAAHAA-NH2 [where “A” indicates a carbon thirteen (13C) "Synthesis was conducted at CPC Scientific Inc. Ac-Asp(OtBu)-Thr(tBu)-His(Trt)-Phe-Pro-Ile-Cys(Trt)-Ile-PhePEG3-Arg(Pbf)-Arg(Pbf)-Lys(Boc)-wang resin (2).. Ac-Asp-Thr-His-Phe-Pro-Ile-Cys-Ile-Phe-PEG3-Arg-Arg-Lys(BODIPY_TMR_C6)."

    Published On: August 1st, 2016Categories: Citations, coauthored, Metal Chelates, PEGylation, publications
  • Engineering highly potent and selective microproteins against Nav1. 7 sodium channel for treatment of pain.”

    Shcherbatko, Anatoly, Andrea Rossi, Davide Foletti, Guoyun Zhu, Oren Bogin, Meritxell Galindo Casas, Mathias Rickert et al. Journal of Biological Chemistry 291, no. 27 (2016): 13974-13986.

    • Rinat Laboratories, Pfizer Inc., South San Francisco, California 94080

    Large scale preparations were performed based on the described protocol by CPC Scientific.

    Published On: July 1st, 2016Categories: Citations
  • Effects of KLK Peptide on Adjuvanticity of Different ODN Sequences

    Chikh, G., Luu, R., Patel, S., Davis, H.L. and Weeratna, R.D. Vaccines 4, no. 2 (2016): 14.

    • Pfizer Vaccine Immunotherapeutics, Ottawa Laboratories, Ottawa, ON K2K 3A2, Canada

    KLK peptide (KLKL5KLK) and KPK peptide (KPKP5KPK) were synthesized by CPC Scientific, San Jose, CA, USA.

    Published On: May 4th, 2016Categories: Citations
  • Mitotic Checkpoint Kinase Mps1 Has a Role in Normal Physiology which Impacts Clinical Utility.

    Martinez, R., Blasina, A., Hallin, J.F., Hu, W., Rymer, I., Fan, J., Hoffman, R.L., Murphy, S., Marx, M., Yanochko, G. and Trajkovic, D. PloS One 10, no. 9 (2015): e0138616.

    • Oncology Research Unit, Pfizer Worldwide Research and Development, 10724 Science Center Drive, San Diego, CA, 92121

    The 60 minute enzymatic reaction contained 5 nM Mps1 catalytic domain (aa 510–857), 60 μM (~Km-level) ATP, 5 μM phosphoacceptor peptide substrate (5FAM-RQRRSIDDTIDSTRLFE-NH2; CPC Scientific, Sunnyvale, CA), 10 mM MgCl2, 4% DMSO (with or without inhibitor), 1 mM DTT, 0.01% Tween-20, 50 mM HEPES (pH 7.5).

    Published On: September 23rd, 2015Categories: Citations, Dye-Labeled
  • Comparison of a High-Throughput Mass Spectrometry Method and Radioactive Filter Binding to Assay the Protein Methyltransferase PRMT5

    Maegley, K.A., Krivacic, C., Bingham, P., Liu, W. and Brooun, A. ASSAY and Drug Development Technologies 13, no. 4 (2015): 235-240.

    • Oncology Research Unit and 2 Oncology Medicinal Chemistry, Pfizer World Wide Research and Development, San Diego, California.

    MS solvents were purchased from Sigma and Burdick & Jackson. Custom peptides were synthesized by CPC Scientific.

    Published On: June 12th, 2015Categories: Citations
  • Axitinib effectively inhibits BCR-ABL1 (T315I) with a distinct binding conformation.

    Pemovska, T., Johnson, E., Kontro, M., Repasky, G.A., Chen, J., Wells, P., Cronin, C.N., McTigue, M., Kallioniemi, O., Porkka, K. and Murray, B.W. Nature 519, no. 7541 (2015): 102-105.

    • La Jolla Laboratories, Pfizer Worldwide Research & Development, San Diego, California 92121, USA.

    The reactions were conducted in 50 ml volumes in 96-well plates and contained GST-tagged human-recombinant ABL1(T315I) kinase intracellular domain (1 nM), 3 mM phosphoacceptor peptide, 59 FAM- EAIYAAPFAKKK-OH (CPC Scientific, also known as ProfilerPro Peptide 2, Caliper Life Sciences), test compound (11-dose threefold serial dilutions, 2% DMSO final) or DMSO only, 1 mM dithiothreitol (DTT), 0.002% Tween-20 and 5 mM MgCl2 in 25 mM HEPES, pH 7.1

    Published On: February 9th, 2015Categories: Citations, Dye-Labeled