"The tandem peptide library used in this work was synthesized via standard FMOC solid-phase peptide synthesis and purified by high-performance liquid chromatography at the MIT Biopolymers Core, Tufts University Core Facility or CPC Scientific, Inc."
Abstract
Aspects of the invention provide compositions and methods for delivering nucleic acids to target cells.
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Espejo, Alexsandra B., et al. Journal of Biological Chemistry 292.6 (2017): 2255-2265.
"PRMT5pT634 (Biotin-SAIHNPTGRSYpTIGL-COOH, where pT represents phosphothreonine), PGHS2 (Biotin-SGSGVLIKRRSTEL-COOH),PGHS2pT602 (Biotin-SGSGVLIKRRSpTEL-COOH), IRK1 (Biotin-SGSGPRPLRRESEI-COOH), IRK1pS425 (BiotinSGSGPRPLRREpSEI-COOH, where pS represents phosphoserine), ERBB4 (Biotin-GTVLPPPPYRHRNTVV-COOH), and ERBB4pT1306 (Biotin-GTVLPPPPYRHRNpTVV-COOH)) and by CPC Scientific, Inc. (E6 (HPV16) (Biotin-RSSRTRRETQLCOOH) and E6 (HPV16)pT156 (Biotin-RSSRTRREpTQLCOOH))."
September 24th, 2016Citations, Phosphorylated PeptidesAyhan, Dilay Hazal, et al. PLoS Biol 14.9 (2016): e1002552.
"The cell-penetrating peptide (RXR)4XB, where R is arginine, X is aminohexanoic acid, and B is beta-alanine, was synthesized using standard FMOC chemistry and purified to >95% purity at CPC Scientific (Sunnyvale, CA) and used without further purification."
September 15th, 2016Citations, Unnatural Amino AcidsLo, Justin H., et al. Bioconjugate Chemistry (2016).
"Myristoyl-transportan−LyP-1 tandem peptide was synthesized by CPC Scientific (sequence: myr-GGWTLNSAGYLLGKINLKALAALAKKIL-GGGG-CGNKRTRGC, Cys−Cys bridge). FAM-labeled LyP-1 bearing an azide (azidoacetyl-GGG-cyclo-(EGNKRTRGK)), FAM-labeled LyP-1 bearing a cysteine (C-K(5FAM)-C6-cyclo-(CGNKRTRGC)) and transportan bearing a C-terminal cysteine (myristic acid-GGWTLNSAGYLLGKINLKALAALAKKILC) were synthesized by CPC Scientific, Inc."
September 1st, 2016Citations, Click Peptides, Dye-Labeled, Lipidation, Peptide MacrocyclesGraaf, Matthew D., et al. ACS Chemical Biology (2016).
"Cu(I)-catalyzed “click” reactions cannot be performed on a borate ester derived polymer coating on a microelectrode array because the Cu(II) precursor for the catalyst triggers background reactions between both acetylene and azide groups with the polymer surface. [..] The new chemistry can be used to explore new biological interactions on the arrays. Specifically, the binding of a v107 derived peptide with both human and murine VEGF was probed using a functionalized microelectrode array."
August 24th, 2016Citations, Click PeptidesHershey, David M., et al. BioRxiv (2016): 047555.
"A custom peptidewith the sequence 5-carboxymethylfluorescein-Thr-Gln-Thr-Val-Ala-Ala-Gly-Ser-Lys(CPQ2)-D-Arg-D-Arg was obtainedcommercially (CPC Scientific)"
August 19th, 2016Citations, D-Amino Acids, Dye-Labeled, FRET SubstratesLaRock, Christopher N., et al. Science Immunology 100.200: 300 (2016).
"Internally quenched peptides of the sequences of IFFDTWDNE, TWDNEAYVH, EAYVHDAPV, and HDAPVRSLN, corresponding to amino acids 103 to 111, 107 to 115, 111 to 119, and 115 to 123 of the reference human pro–IL-1b sequence (UniProt: P01584), were labeled on the N terminus with Mca and on the C terminus with Lys-Dnp (CPC Scientific)."
August 19th, 2016Citations, FRET SubstratesChen, Long, et al. Scientific Reports 6 (2016).
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Peking University, Beijing 100871, China
- Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA
- School of Life Sciences, Nanjing University, China
- Peking-Tsinghua Center for Life Sciences, Beijing, China
"The Biotin-C6-LELPETGG-NH2, GGGY-Lys(Biotin)-NH2, and GGG-Lys(N3)-NH2 reagents were synthesized by CPC Scientific."
August 18th, 2016Citations, Click Peptides, Unnatural Amino AcidsGoupil, Louise S., et al. PLoS Negl Trop Dis 10.8 (2016): e0004893.
"Protease activity in regurgitant from three replicate tanks were compared using a mixture of 7 internally quenched fluorescent substrates available from CPC Scientific, Sunnyvale, California (Table 1) [AMYD-112, AMYD-114, AMYD-109, MMPS-024, SUBS-017, AMYD-111, CASP-060]. These substrates were chosen based on their diverse sequence composition to enable detection of multiple protease classes."
August 8th, 2016Citations, FRET SubstratesGarner, Thomas P., et al. Molecular Cell 63.3 (2016): 485-497.
"Hydrocarbon-stapled peptide corresponding to the BH3 domain of BIM, BIM SAHBA: N-acetylated 145EIWIAQELRS5IGDS5FNAYYA164-CONH2, where S5 represents the non-natural amino acid inserted for olefin metathesis, was synthesized, purified and characterized as previously described by CPC Scientific (Gavathiotis et al., 2008)."
August 4th, 2016Citations, Stapled Peptides