"Folded HD5 was made from a synthesized 80% pure linearized peptide (CPC Scientific, Sunnyvale, CA) and purified by reverse-phase high-pressure liquid chromatography, and an HD5 derivative containing L-α-aminobutyric acid in place of cysteine (HD5 Abu) was chemically synthesized, as previously described.."
Abstract
Human papillomavirus (HPV) is a significant oncogenic virus, but the innate immune response to HPV is poorly understood. Human α-defensin 5 (HD5) is an innate immune effector peptide secreted by epithelial cells in the genitourinary tract. HD5 is broadly antimicrobial, exhibiting potent antiviral activity against HPV at physiologic concentrations; however, the specific mechanism of HD5-mediated inhibition against HPV is unknown. During infection, the HPV capsid undergoes several critical cell-mediated viral protein processing steps, including unfolding and cleavage of the minor capsid protein L2 by host cyclophilin B and furin. Using HPV16 pseudovirus, we show that HD5 interacts directly with the virus and inhibits the furin-mediated cleavage of L2 at the cell surface during infection at a step downstream of the cyclophilin B-mediated unfolding of L2. Importantly, HD5 does not affect the enzymatic activity of furin directly. Thus, our data support a model in which HD5 prevents furin from accessing L2 by occluding the furin cleavage site via direct binding to the viral capsid.
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Willard, F.S., Wainscott, D.B., Showalter, A.D., Stutsman, C., Ma, W., Cardona, G.R., Zink, R.W., Corkins, C.M., Chen, Q., Yumibe, N. and Agejas, J. Journal of Medicinal Chemistry 64, no. 6 (2021): 3439-3448
- Discovery Chemistry Research and Technologies, Diabetes and Complications, Investigative Drug Disposition, Lilly Research Laboratories, Indianapolis, IN 46285 and S2.
- Discovery Chemistry Research and Technologies, Lilly, S.A., Avda. de la Industria 30, 28108 Alcobendas, Madrid, Spain.
Synthetic peptides were synthesized at Eli Lilly, Bachem (Torrance, CA), or CPC Scientific (Sunnyvale, CA) to >95% purity.
Libiger, O., Shaw, L.M., Watson, M.H., Nairn, A.C., Umaña, K.L., Biarnes, M.C., Canet‐Avilés, R.M., Jack Jr, C.R., Breton, Y.A., Cortes, L. and Chelsky, D. Alzheimer's & Dementia 17, no. 12 (2021): 1976-1987.
- Janssen Research and Development, 3210 Merryfield Row, San Diego, CA 92121, USA.
- Foundation for the National Institutes of Health, North Bethesda, Maryland, USA
- Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA
- Merck & Co., Inc., West Point, Pennsylvania, USA
Purified synthetic stable isotope (15N and 13C) labeled (SIL) peptides were from CPC Scientific (EDSLEAGLPLQVR [CMGA]; SLGVGFATR [FABPH]; VLEYLNQEK [SCG2]; and THLGEALAPLSK [VGF]); and JPT Peptide Technologies (TNYLYGK [NPTX2]).
Alford, M. A.; Choi, K.-Y. G.; Trimble, M. J.; Masoudi, H.; Kalsi, P.; Pletzer, D.; Hancock, R. E., Frontiers in Cellular and Infection Microbiology 2021, 2021, 11, 75.
"Peptides IDR-1018 (VRLIVAVRIWRR-NH2) (Achtman et al., 2012), DJK-5 (all D-amino acids vqwrairvrvir-NH2) (de la Fuente-Nunez et al., 2015) and IDR-1002 (VQRWLIVWRIRK-NH2) (Nijnik et al., 2010), were synthesized by CPC Scientific [..]"
GhavamiNejad, A.; Lu, B.; Samarikhalaj, M.; Liu, J. F.; Mirzaie, S.; Pereira, S.; Zhou, L.; Giacca, A.; Wu, X. Y., Drug Delivery and Translational Research 2021, 1-13.
"PRL-2903 (PRL) was synthesized and purchased from CPC Scientific Inc. (San Jose, CA, USA)."
Spitz, A. Z.; Zacharioudakis, E.; Reyna, D. E.; Garner, T. P.; Gavathiotis, E., Nature Communications 2021, 12 (1), 1-15.
Hydrocarbon-stapled peptides corresponding to the BH3 domain of BIM, BIM SAHB: FITC-Ahx-EIWIAQELRS5IGDS5FNAYYA-CONH, where S5 represents the non-natural amino acid inserted for olefin metathesis, were synthesized and purified at >95% purity by CPC Scientific Inc.
Zeming, K. K.; Lu, R.; Woo, K. L.; Sun, G.; Quek, K. Y.; Cheow, L. F.; Chen, C.-H.; Han, J.; Lim, S. L. Analytical Chemistry 2021.
The FRET substrates used in this project are: neutrophil elastase substrate ((Z-AAAA)2Rh110 Abs/Em:497/520 nm, Anaspec), Granzyme B substrate (5TAMRA-VGPDFGR-K(QSY7)-NH2 Abs/Em: 546/580, CPC scientific), and metalloproteinase substrate (QSY21-HGDQMAQKSK(Cy5)-NH2 Abs/Em:649/666 nm, CPC Scientific).
2020 has been a challenging year for all of us. We have experienced firsthand how a global pandemic has changed the world we live in. In spite of this, we’ve seen people and business coming together to make a difference. CPC Scientific’s mission is to collaborate with our customers to improve the quality of […]
DePalma, S. J.; Davidson, C. D.; Stis, A. E.; Helms, A. S.; Baker, B. M., Biomaterials Science, 2021, 9 (1), 93-107.
.. matrices were functionalized with cell adhesive peptides.. Gly-Phe-Hyp-Gly-Glu-Arg (GFOGER; CPC Scientific) via Michael-Type addition to available vinyl sulfone groups."
Zonari, A.; Brace, L. E.; Al-Katib, K. Z.; Porto, W. F.; Foyt, D.; Guiang, M.; Cruz, E. A. O.; Marshall, B.; Salgueiro, W. G.; Inan, M. D., bioRxiv 2020, 2020.10. 30.362822.
The top hits peptides selected (peptide 14, 144, 156, 195, and 393) from the screening were purchased from CPC Scientific Inc.