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      1. Home
      2. Posts
      3. Multiple Disulfide Bridges

      Multiple Disulfide Bridges Archive

      • Neuropilin-1 drives tumor-specific uptake of chlorotoxin.

        McGonigle, Sharon, Utpal Majumder, Donna Kolber-Simonds, Jiayi Wu, Andrew Hart, Thomas Noland, Karen TenDyke et al. Cell Communication and Signaling 17, no. 1 (2019): 67.

        Native Cltx (Cltx-CONH2) was synthesized at CPC Scientific (Sunnyvale, CA) by routine methods and stored dry at -20 °C.

        Published On: June 17th, 2019Categories: Citations, Multiple Disulfide Bridges
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      • In vitro reconstitution of Wnt acylation reveals structural determinants of substrate recognition by the acyltransferase human Porcupine.

        Lee, Chul-Jin, Mitra S. Rana, Chanhyung Bae, Yan Li, and Anirban Banerjee. Journal of Biological Chemistry 294, no. 1 (2019): 231-245.

        Substrate-mimicking peptides for wild-type or mutant Wnts with a point-mutation, varying in lengths or the number of disulfide bonds were purchased from CPC Scientific (Sunnyvale, CA)..

        Published On: November 12th, 2018Categories: Citations, Multiple Disulfide Bridges
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      • Alpha-defensin-dependent enhancement of enteric viral infection.

        Wilson, Sarah S., et al. PLoS Pathogens 13.6 (2017): e1006446.

        "Cryptdin 2 (UniProtKB: P28309, LRDLVCYCRTRGCKRRERMNGTCRKGHLMYTLCCR)... obtained by oxidative refolding of partially purified linear peptides (synthesized by CPC Scientific...) and purifying the correctly folded species by reverse-phase high-pressure liquid chromatography (RP-HPLC). Purity was determined by analytical RP-HPLC, and the mass of the disulfide-bonded peptides was verified by high mass accuracy liquid chromatography-mass spectrometry."

        Published On: June 16th, 2017Categories: Antimicrobial Peptides, Citations, Multiple Disulfide Bridges
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      • Molecular and serologic markers of HPV 16 infection are associated with local recurrence in patients with oral cavity squamous cell carcinoma.

        Huang, Chung-Guei, et al. Oncotarget 8.21 (2017): 34820-34835

        "Synthetic peptides of HPV 16 L1 (N′-C-KHTPPAPKEDPLKK-C′; position: 456−471)/E6 (N′-C-RTAMFQDPQERPRK-C′; position: 5−18) and a recombination protein of full-length HPV 16 E7-histag fusion protein (N′-MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEED-EIDGPAGQAEPDRAHYNIVTFCCKCDSTLRL-CVQSTHVDIRTLEDLLMGTLGIVCPICSQKP-C′; position: 1−98) were manufactured by CPC Scientific Inc."

        Published On: March 31st, 2017Categories: Antimicrobial Peptides, Long Sequences, Multiple Disulfide Bridges
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      • Alpha-defensin HD5 inhibits furin cleavage of human papillomavirus 16 L2 to block infection

        Wiens, Mayim E., and Jason G. Smith. "Alpha-defensin HD5 inhibits furin cleavage of human papillomavirus 16 L2 to block infection." Journal of Virology 89, no. 5 (2015): 2866-2874.

        "Folded HD5 was made from a synthesized 80% pure linearized peptide (CPC Scientific, Sunnyvale, CA) and purified by reverse-phase high-pressure liquid chromatography, and an HD5 derivative containing L-α-aminobutyric acid in place of cysteine (HD5 Abu) was chemically synthesized, as previously described.."

        Published On: February 5th, 2015Categories: Citations, Multiple Disulfide Bridges
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      • A small intestinal organoid model of non-invasive enteric pathogen–epithelial cell interactions.

        Wilson, Sarah S., et al. Mucosal Immunology 8.2 (2015): 352-361.

        "Folded Crp23 was created from a synthesized 80% pure linear peptide (CPC Scientific, Sunnyvale, CA) by the same procedure as previously reported for the α-defensin HD5"

        Published On: August 13th, 2014Categories: Antimicrobial Peptides, Citations, Multiple Disulfide Bridges
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      • Critical determinants of human α-defensin 5 activity against non-enveloped viruses.

        Gounder, Anshu P., et al. Journal of Biological Chemistry 287.29 (2012): 24554-24562.

        "..folded HD5 was generated from a synthesized 80% pure linear peptide (CPC Scientific, Sunnyvale, CA) by thiol disulfide reshuffling overnight at room temperature in the presence of 3 mM reduced and 0.3 mM oxidized glutathione, 2 M guanidine hydrochloride, and 0.25 M sodium bicarbonate, pH 8.3, at a peptide concentration of 0.25 mg/ml [..] All α-defensins were quantified by UV absorbance at 280 nm using calculated molar extinction coefficients (18)."

        Published On: May 25th, 2012Categories: Antimicrobial Peptides, Citations, Multiple Disulfide Bridges
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      • Electropositive charge in α-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure.

        Llenado, R. Alan, et al. Infection and Immunity 77.11 (2009): 5035-5043.

        "(R/K)-RMAD-4 [rhesus myeloid α-defensin 4] [(R1/2/5/7/10/13/14/26/33K)-RMAD-462-94] was custom synthesized by CPC Scientific, Inc. (San Jose, CA) and refolded as described previously…"

        Published On: October 16th, 2009Categories: Antimicrobial Peptides, Citations, Multiple Disulfide Bridges
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